Invention Grant
- Patent Title: Protein glycosylation modification in methylotrophic yeast
- Patent Title (中): 甲基营养酵母中的蛋白质糖基化修饰
-
Application No.: US12424924Application Date: 2009-04-16
-
Publication No.: US08663971B2Publication Date: 2014-03-04
- Inventor: Roland Contreras , Nico L. M. Callewaert , Steven C. J. Geysens
- Applicant: Roland Contreras , Nico L. M. Callewaert , Steven C. J. Geysens
- Applicant Address: BE Zwijnaarde US AZ Tucscon BE Ghent
- Assignee: VIB, VZW,Research Corporation Technologies, Inc.,Universiteit Gent
- Current Assignee: VIB, VZW,Research Corporation Technologies, Inc.,Universiteit Gent
- Current Assignee Address: BE Zwijnaarde US AZ Tucscon BE Ghent
- Agency: Scully, Scott, Murphy & Presser, P.C.
- Main IPC: C12N1/14
- IPC: C12N1/14 ; C12N15/74 ; C12P21/04 ; C12N15/00
Abstract:
The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an α-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.
Public/Granted literature
- US20100267084A1 PROTEIN GLYCOSYLATION MODIFICATION IN METHYLOTROPHIC YEAST Public/Granted day:2010-10-21
Information query
IPC分类:
