A polymerase chain reaction (“PCR”) apparatus includes a PCR chip holder which accommodates and supports a PCR chip, a housing which supports ends of the PCR chip holder, a temperature control element which moves perpendicularly with respect to the PCR chip holder in a space between the housing and the PCR chip holder in the housing, and an elastic unit which elastically biases the temperature control element toward the PCR chip holder, between the temperature control element and the housing. The temperature control element includes a top surface which is selectively contacted to a bottom surface of the PCR chip.