Invention Grant
- Patent Title: Method for determining pectin content in plant sample
- Patent Title (中): 测定植物样品中果胶含量的方法
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Application No.: US13806101Application Date: 2011-12-12
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Publication No.: US08669068B2Publication Date: 2014-03-11
- Inventor: Haohui Kong , Yifei Huang , Baofeng Jin
- Applicant: Haohui Kong , Yifei Huang , Baofeng Jin
- Applicant Address: CN Guangdong
- Assignee: China Tobacco Guangdong Industrial Co., Ltd.
- Current Assignee: China Tobacco Guangdong Industrial Co., Ltd.
- Current Assignee Address: CN Guangdong
- Agency: Muncy, Geissler, Olds & Lowe, P.C.
- Priority: CN201110007055 20110113
- International Application: PCT/CN2011/083859 WO 20111212
- International Announcement: WO2012/094934 WO 20120719
- Main IPC: C12Q1/34
- IPC: C12Q1/34 ; C12P7/06 ; C12M1/36 ; C12M1/38 ; C12M3/00 ; C12M1/34 ; C12M1/00

Abstract:
A method for determining pectin content in a plant sample includes: 1) adding an acidic alcohol solution to the plant sample, heating in a water bath and filtration; 2) soaking residue from step 1 with an acidic solution, heating in a water bath, a second filtration, bringing to volume after cooling and obtaining filtrate; 3) adding an acetic acid/sodium acetate buffer solution to residue of step 2, adding a pectinase solution, heating under vibration in a water bath, and a third filtration to obtain a filtrate; 4) adding an acetic acid/sodium acetate buffer solution and a pectinase solution to filtrate from step 2, heating the mixture under vibration in a water bath to obtain an enzymatic hydrolysate, adding filtrate obtained in step 3 to the enzymatic hydrolysate, bringing to volume, and obtaining a test solution; 5) drawing the test solution into a continuous flow analyzer to perform analysis.
Public/Granted literature
- US20130143249A1 METHOD FOR DETERMINING PECTIN CONTENT IN PLANT SAMPLE Public/Granted day:2013-06-06
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