Invention Grant
- Patent Title: Enhanced pyruvate to acetolactate conversion in yeast
- Patent Title (中): 增强酵母中的丙酮酸至乙酰乳酸转化
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Application No.: US13299954Application Date: 2011-11-18
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Publication No.: US08669094B2Publication Date: 2014-03-11
- Inventor: Larry Cameron Anthony , Lori Ann Maggio-Hall
- Applicant: Larry Cameron Anthony , Lori Ann Maggio-Hall
- Applicant Address: US DE Wilmington
- Assignee: Butamax™ Advanced Biofuels LLC
- Current Assignee: Butamax™ Advanced Biofuels LLC
- Current Assignee Address: US DE Wilmington
- Agent Christine M. Lhulier
- Main IPC: C12N9/88
- IPC: C12N9/88 ; C12N1/19 ; C12P7/04
Abstract:
A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
Public/Granted literature
- US20120252084A1 Enhanced Pyruvate to Acetolactate Conversion in Yeast Public/Granted day:2012-10-04
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