Invention Grant
US08669109B2 Methods of producing proteins in Chinese hamster ovary (CHO) cells
有权
在中国仓鼠卵巢（CHO）细胞中产生蛋白质的方法
- Patent Title: Methods of producing proteins in Chinese hamster ovary (CHO) cells
- Patent Title (中): 在中国仓鼠卵巢（CHO）细胞中产生蛋白质的方法
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Application No.: US12899914Application Date: 2010-10-07
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Publication No.: US08669109B2Publication Date: 2014-03-11
- Inventor: Ralf Otto , Barbara Enenkel , Juergen Fieder , Thomas Krieg
- Applicant: Ralf Otto , Barbara Enenkel , Juergen Fieder , Thomas Krieg
- Applicant Address: DE Ingelheim
- Assignee: Boehringer Ingelheim Pharma GmbH & Co. KG
- Current Assignee: Boehringer Ingelheim Pharma GmbH & Co. KG
- Current Assignee Address: DE Ingelheim
- Agent Michael P. Morris; Edouard G. Lebel
- Priority: DE10338531 20030819
- Main IPC: C12N15/00
- IPC: C12N15/00 ; C12N5/07 ; C12N5/10 ; C12N5/00 ; C12N5/02
Abstract:
A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.
Public/Granted literature
- US20110250640A1 METHOD OF RECLONING PRODUCTION CELLS Public/Granted day:2011-10-13
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