A sealing structure is provided wherein an annular groove formed in a pump cylinder of a piston pump receives therein a seal ring made of a synthetic resin material and having an inner circumferential surface fluid-tightly contacting an outer circumferential surface of the pump piston and an O-ring arranged on the outer circumferential side of the seal ring and urging the seal ring radially inward. The seal ring is formed at its axial end portions with flange portions for preventing the O-ring from coming off. In the state that the seal ring and the O-ring are fitted in the annular groove, the flange portions do not receive a load from the O-ring, and a surface pressure which the seal ring applies to the outer circumferential surface of the piston at an axial center portion thereof is set to be higher than surface pressures which it applies to the outer circumferential surface of the piston at axial end portions thereof.