Invention Grant
US08685645B2 Screening method and vector, vector library, and assay kit used therefor
有权
筛选方法和载体，载体文库和用于其的测定试剂盒
- Patent Title: Screening method and vector, vector library, and assay kit used therefor
- Patent Title (中): 筛选方法和载体，载体文库和用于其的测定试剂盒
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Application No.: US12886642Application Date: 2010-09-21
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Publication No.: US08685645B2Publication Date: 2014-04-01
- Inventor: Eiichi Akahoshi , Mitsuko Ishihara
- Applicant: Eiichi Akahoshi , Mitsuko Ishihara
- Applicant Address: JP Tokyo
- Assignee: Kabushiki Kaisha Toshiba
- Current Assignee: Kabushiki Kaisha Toshiba
- Current Assignee Address: JP Tokyo
- Agency: Oblon, Spivak, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2010-082873 20100331
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12N15/00 ; C12N15/64
Abstract:
According to one embodiment, a method of screening an enhancer and/or a promoter, includes culturing a host cell into which an amplifiable vector is introduced, extracting the vector from the host cell and obtaining the DNA fragment from the extracted vector, wherein the vector includes a DNA fragment to be determined, a gene that is functionally linked downstream of the DNA fragment and encodes a protein to initiate self-replication, and a gene that encodes a replication origin sequence.
Public/Granted literature
- US20110244471A1 SCREENING METHOD AND VECTOR, VECTOR LIBRARY, AND ASSAY KIT USED THEREFOR Public/Granted day:2011-10-06
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