Invention Grant
US08685671B2 Process for producing recombinant glycoproteins by culturing a Hansenula polymorpha mutant strain
有权
通过培养汉逊酵母多形汉逊酵母突变菌株来生产重组糖蛋白的方法
- Patent Title: Process for producing recombinant glycoproteins by culturing a Hansenula polymorpha mutant strain
- Patent Title (中): 通过培养汉逊酵母多形汉逊酵母突变菌株来生产重组糖蛋白的方法
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Application No.: US13451223Application Date: 2012-04-19
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Publication No.: US08685671B2Publication Date: 2014-04-01
- Inventor: Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
- Applicant: Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
- Applicant Address: KR Daejeon
- Assignee: Korea Research Institute of Bioscience and Biotechnology
- Current Assignee: Korea Research Institute of Bioscience and Biotechnology
- Current Assignee Address: KR Daejeon
- Agency: Lucas & Mercanti, LLP
- Priority: KR10-2005-0101992 20051027
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C12N15/81 ; C12N15/90 ; C12N1/19 ; C12N9/10
Abstract:
The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
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