Invention Grant
US08685679B2 Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
失效
酰基转移酶调节以增加含油生物的总脂质和油中多不饱和脂肪酸的百分比
- Patent Title: Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
- Patent Title (中): 酰基转移酶调节以增加含油生物的总脂质和油中多不饱和脂肪酸的百分比
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Application No.: US11190750Application Date: 2005-07-27
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Publication No.: US08685679B2Publication Date: 2014-04-01
- Inventor: Stephen K. Picataggio , Narendra S. Yadav , Hongxiang Zhang
- Applicant: Stephen K. Picataggio , Narendra S. Yadav , Hongxiang Zhang
- Applicant Address: US DE Wilmington
- Assignee: E I du Pont de Nemours and Company
- Current Assignee: E I du Pont de Nemours and Company
- Current Assignee Address: US DE Wilmington
- Main IPC: C12P7/64
- IPC: C12P7/64 ; C12Q1/48 ; C12N1/00 ; C12N9/10 ; C07H21/04
Abstract:
Methods to increase the percent of polyunsaturated fatty acids (PUFAs) within the total lipids and oils of PUFA-producing oleaginous organisms are provided herein, by regulating the activity of specific acyltransferases. Specifically, since oil biosynthesis is expected to compete with polyunsaturation during oleaginy, it is possible to reduce or inactivate the activity of an organism's DAG ATs (e.g., phospholipid:diacylglycerol acyltransferase (PDAT) and/or diacylglycerol acyltransferase 1 (DGAT1) and/or diacylglycerol acyltransferase 2 (DGAT2)) to thereby reduce the overall rate of oil biosynthesis while concomitantly increasing the percent of PUFAs that are incorporated into the lipid and oil fractions. The teachings herein will thereby enable one to engineer a wide variety of oleaginous organisms to produce oils with very specific fatty acid compositions.
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