A spectral imaging system for collecting spectral information of a two dimensional heterogeneous objects while in motion relative to the imaging system without the use of a spectrograph, filters or any dispersive optics. The system includes a pulsed light source tunable in wavelength for producing short pulses of wavelength tuned light at a plurality of selected narrow band wavelengths within a spectral range and one or more optical components for conveying or directing the short pulses of light to a two dimensional region that is substantially stationary with respect to the imaging system and through which the two dimensional target is moving. The system also includes a many pixel camera synchronized with the tunable pulsed light source. The camera is adapted to detect light in the selected narrow band wavelengths produced by the tunable pulsed light source and reflected from or transmitted through the two dimensional target in motion relative to the imaging system and to provide image frame information with each frame at a defined wavelength band. A processor processes information from said tunable light source and said camera and to produce multi-spectral images of the target.