A biomedical diagnostic device includes a hydrophilic layer, hydrophobic layer, and at least one test pad. The hydrophilic layer includes an exposed introductory portion adapted to introduce a test fluid, and is stacked with the hydrophobic layer with multiple access holes. At least one test pad, each of which includes a distinguishing reaction medium, is disposed in the corresponding holes that extend to contact the hydrophilic layer. The relations within the device are as follows: hydrophilicity of any of the test pads>hydrophilicity of the hydrophilic layer>hydrophilicity of the hydrophobic layer.