This invention provides quantitative, systematic and standardized High Throughput Screening (HTS) methods capable to the identification of both known and unknown sequences without prior knowledge of the sequences of interest. Either coding or non-coding sequences could be targeted, identified and analyzed systematically and respectively at genomic, transcriptional and translational levels with those methods. The genetic algorithms for sequence deducing and standardized universal libraries constructing are provided. The genetic algorithms are 61.sup.(n−m), 61.sup.n, 64.sup.(n−m), 64.sup.n., 20.sup.(n−m) and 20.sup.n. Applications of the standardized universal libraries include gene expression profiling, signature sequence identification and sequence determination by PCR, cloning, dot-blot hybridization, ELISA, DNA and Peptide Arrays.