The present invention relates to an RFID-based intelligent storage cabinet and its management method, while utilizing the shielding effect enabled by each partitions for allowing any electromagnetic wave in one compartment to transmit only inside the confinement of such compartment without interfering another compartment. Each stock keeping unit (SKU) that is to be stored in the RFID-based intelligent storage cabinet is attached with at least one RFID tag, and different SKUs of different usages are sorted so as to be stored in different compartments. Moreover, each RFID reader can be designed to function according to the command of a control device for scanning the corresponding compartment in a periodic manner or non-periodic manner, by that any SKU that is stored in the corresponding compartment can be detected and identified as each RFID reader is also connected to an inventory database, a database of item list, or a memory database.