A human fatigue assessment device capable of performing highly accurate fatigue assessment is provided. The human fatigue assessment device includes: a physiological signal measuring unit which measures a pulse wave signal of a user; a feature value extracting unit which extracts first feature values each of which is obtained from a systolic posterior component of the pulse wave signal measured by the physiological signal measuring unit; a storage unit in which the first feature values extracted by the feature value extracting unit are stored; and a fatigue determining unit which determines whether or not the user is fatigued, using the first feature values extracted by the feature value extracting unit, in which the fatigue determining unit compares a first feature value among the first feature values extracted by the feature value extracting unit and at least one of the first feature values stored in the storage unit, to determine whether or not the user is fatigued.