A tool holder includes a drawing screw and a stopper member. The drawing screw is positioned in an intermediate hole. A rear end face of a head of the drawing screw faces a boundary wall surface, and a top end of a shaft of the drawing screw is screwed in a rear end of a collet. The stopper member is positioned in an annular space between an outer peripheral surface of the shaft of the drawing screw and an inner peripheral surface of the intermediate hole. The stopper member faces a front end face of the head of the drawing screw, and limits movement of the head toward a top end of the tool holder. The drawing screw and the stopper member are inserted and assembled from a top end opening of a collet holding hole.