A glucose dehydrogenase, having high substrate specificity, can be produced at a low cost, is not affected by oxygen dissolved in a measurement sample and, in particular, has superior thermal stability is obtained by culturing a microorganism belonging to the genus Burkholderia, particularly Burkholderia cepacia. The glucose dehydrogenase produced by the microorganism is collected from the medium and/or from the cells of the microorganism.