Invention Grant
- Patent Title: Amplification and analysis of selected targets on solid supports
- Patent Title (中): 固体支持物上选定靶标的扩增和分析
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Application No.: US12899540Application Date: 2010-10-06
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Publication No.: US08716190B2Publication Date: 2014-05-06
- Inventor: Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
- Applicant: Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
- Applicant Address: US CA Santa Clara
- Assignee: Affymetrix, Inc.
- Current Assignee: Affymetrix, Inc.
- Current Assignee Address: US CA Santa Clara
- Agency: Affymetrix, Inc.
- Main IPC: C40B30/04
- IPC: C40B30/04

Abstract:
Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.
Public/Granted literature
- US20110269631A1 Amplification and Analysis of Selected Targets on Solid Supports Public/Granted day:2011-11-03
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