A repair amplification circuit includes a controlling unit, a first operational amplifier, a second operational amplifier, a first switching unit, and a second switching unit. Under a detection mode, the controlling unit generates a detecting signal according to a testing signal transmitted by a unnecessary repair segment in a test picture. Under an operation mode, the controlling unit generates a switching signal according to a repair controlling signal related to the detecting signal. The first switching unit and the second switching unit are controlled by the switching signal, so as to transmit the driving signal to one of the first operational amplifier and the second operational amplifier, and to transmit a positive polarity repairing signal generated by the first operational amplifier or a negative polarity repairing signal generated by the second operational amplifier to a necessary repair segment.