A stereoscopic optical device includes a substrate, an alignment film and a liquid crystal layer. The alignment film includes at least one first region and at least one second region. The liquid crystal layer, disposed on the alignment film, includes first liquid crystal molecules and second liquid crystal molecules. The first liquid crystal molecules correspond to the first region of the alignment film and have a first pre-tilt angle, the second liquid crystal molecules correspond to the second region of the alignment film and have a second pre-tilt angle. The fast axis of the first liquid crystal molecules and the fast axis of the second crystal molecules substantially face the same direction, and the slow axis of the first liquid crystal molecules and the slow axis of the second liquid crystal molecules substantially face in the same direction.