An OPO is disclosed capable of rapid frequency tuning by non-mechanical means. The OPO includes a resonant cavity including one or more non-linear crystals in an optical path thereof. A pump laser pulse is transmitted into the resonant cavity simultaneously with a seed beam having a desired wavelength. The output beam from the resonant cavity has the same center wavelength as the seed beam. The wavelength of the seed beam may be modulated at a frequency larger than the pulse rate of the pump laser or larger than the inverse of the pulse duration. The OPO disclosed may be used to perform DIAL analysis wherein intra-pulse modulation of an output beam is used to obtain measurements of absorption at multiple frequencies for each pulse of a pump beam.