A method of transmitting a frame of a relay station (RS) in a wireless communication system employing the RS is provided. The method includes: receiving frame configuration information on an RS frame from a base station (BS); configuring a frame including a downlink (DL) access zone for transmitting a signal to a relay user equipment (UE), a DL receive zone for receiving a signal from the BS, an uplink (UL) access zone for receiving a signal from the relay UE connected to the RS, a UL transmit zone for transmitting a signal to the BS, and a transition gap; and transmitting a signal in at least one of the UL access zone and the UL transmit zone, wherein the transition gap is a switching time between a transmission operation and a reception operation of the RS and is included in at least one of the DL access zone and the UL transmit zone.