Invention Grant
- Patent Title: Protein multi-blotting method and device
- Patent Title (中): 蛋白多点印迹法及装置
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Application No.: US13282030Application Date: 2011-10-26
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Publication No.: US08721860B2Publication Date: 2014-05-13
- Inventor: Stanley Yung-Ping Chien , Hiroki Yokota
- Applicant: Stanley Yung-Ping Chien , Hiroki Yokota
- Applicant Address: US IN Indianapolis
- Assignee: Indiana University Research and Technology Corporation
- Current Assignee: Indiana University Research and Technology Corporation
- Current Assignee Address: US IN Indianapolis
- Agency: Brannon Sowers & Cracraft PC
- Agent C. John Brannon
- Main IPC: G01N27/447
- IPC: G01N27/447

Abstract:
A technique for producing multiple protein blots from a single gel, including entering the number ‘n’ membranes to be blotted into microprocessor memory, determining calibration constants for a gel batch, inputting calibration constants into microprocessor memory, and calculating ‘n’ voltage/time profiles for simultaneous blotting. A gel layer from the gel batch is treated with a protein sample, ‘n’ membranes are placed onto the gel layer to yield a gel pack, and the gel pack is placed between an electrode plate maintained at a fixed first voltage and an array of ‘n’ spaced generally parallel electrodes. The voltage to each respective ‘n’ spaced generally parallel electrodes is varied over time according to a respective ‘n’ voltage/time profile to transfer proteins to each respective ‘n’ membranes to yield blotted membranes to yield ‘n’ respective blotted membranes.
Public/Granted literature
- US20120103811A1 PROTEIN MULTI-BLOTTING METHOD AND DEVICE Public/Granted day:2012-05-03
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