A zeolite membrane excellent in separation performance and having an acid resistance, and a separation membrane employing the zeolite membrane are provided. A zeolite membrane comprising a layer wherein at least two different types of zeolite crystals are present in mixed state, wherein at least two types of the above at least two different types of zeolite crystals are detectable by an X-ray pattern obtained by X-ray diffraction under the conditions that (A) the output of X-ray is 1.2 kW, (B) an X-ray bulb of copper (Cu) is employed, and (C) the wavelength of X-ray is 1.54058 A. As an alternative, the zeolite membrane comprising a first layer containing zeolite crystals and a second layer containing different type of zeolite crystals from the zeolite crystals of the first layer, wherein the first layer and the second layer constitute a laminate structure, and the thickness of the laminate structure is at most 20 pm.