A donor substrate may include a base substrate, a light-to-heat conversion layer, a transfer layer, and a rib structure. The light-to-heat conversion layer may be on the base substrate. The transfer layer may be on the light-to-heat conversion layer. The rib structure is on the transfer layer. The rib structure may include a plurality of tubes spaced apart from one another. In a laser induced thermal imaging process, the donor substrate including the rib structure may be easily removed from a display substrate without damage, thereby to form an organic layer pattern regularly on the display substrate.