A disposable flexible multi-panel sterilization assembly incorporating an integrated fastening system. The disposable flexible multi-panel sterilization assembly includes a barrier panel configured to be folded around content to be sterilized to form a package. The assembly further includes a fold protection panel having a proximal end adjacent the barrier panel and a distal end such that the distal end covers the one or more panel edges of the barrier panel after the barrier panel is in the folded configuration. The integrated fastening system is a barrier panel attachment means located on a portion of the barrier panel for securing one or more panel edges of the barrier panel in a folded configuration around content to be sterilized. The barrier panel attachment means are configured to secure the one or more panel edges in a folded configuration.