Invention Grant
US08728789B2 DNA fragment encoding a polyphosphate-driven nucleoside 5′-diphosphate kinase polypeptide 有权
编码多磷酸盐驱动的核苷5'-二磷酸激酶多肽的DNA片段

DNA fragment encoding a polyphosphate-driven nucleoside 5′-diphosphate kinase polypeptide
Abstract:
The invention provides a method for producing 3′-phosphoadenosine 5′-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5′-triphosphate sulfurylase (ATPS) and adenosine 5′-phosphosulfate kinase (APSK), wherein an adenosine 5′-triphosphate (ATP) supply/regeneration system including adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5′-triphosphate (ATP) supply/regeneration system including adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.
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