Invention Grant
- Patent Title: Method for facilitating an automated isolation of a biopolymer using magnetic particles
- Patent Title (中): 促进使用磁性颗粒自动分离生物聚合物的方法
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Application No.: US10567351Application Date: 2004-08-16
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Publication No.: US08729252B2Publication Date: 2014-05-20
- Inventor: Ralf Himmelreich , Sabine Werner
- Applicant: Ralf Himmelreich , Sabine Werner
- Applicant Address: DE
- Assignee: Qiagen GmbH
- Current Assignee: Qiagen GmbH
- Current Assignee Address: DE
- Agency: McDermott Will & Emery LLP
- Priority: EP03019746 20030829
- International Application: PCT/EP2004/009156 WO 20040816
- International Announcement: WO2005/021748 WO 20050310
- Main IPC: C07H21/00
- IPC: C07H21/00
Abstract:
A method for isolating polynucleotides, such as DNA, RNA and hybrids thereof from an aqueous solution containing polynucleotides by reversibly binding the polynucleotides to silica-coated magnetic particles in the presence of a salt and non-ionic hydratable additive is disclosed. The salt and non-ionic hydratable additive concentrations are adjusted to levels that result in adhesion of the nucleic acid to the particles without degradation or precipitation of the nucleic acid.
Public/Granted literature
- US20080132694A1 Method For Facilitating an Automated Isolation of a Biopolymer Using Magnetic Particles Public/Granted day:2008-06-05
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