Invention Grant
- Patent Title: Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
- Patent Title (中): 将目的核苷酸序列靶向插入植物基因组的组合物和方法
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Application No.: US12039307Application Date: 2008-02-28
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Publication No.: US08735158B2Publication Date: 2014-05-27
- Inventor: Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura Tagliani
- Applicant: Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura Tagliani
- Applicant Address: US IA Johnston
- Assignee: Pioneer Hi-Bred International, Inc.
- Current Assignee: Pioneer Hi-Bred International, Inc.
- Current Assignee Address: US IA Johnston
- Agency: Ballard Spahr LLP
- Main IPC: A01H1/00
- IPC: A01H1/00 ; C12N15/90 ; C12N15/82 ; C07H21/04
Abstract:
Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
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