An optical fiber holder includes a pair of first optical fiber guides, each of the first optical fiber guides satisfying a condition of an allowable bend radius of an optical fiber; a second optical fiber guide configured to hold a joined portion of the optical fiber at a position between the first optical fiber guides, the second optical fiber guide satisfying the condition of the allowable bend radius of the optical fiber; and a tray to hold the first optical fiber guides and the second optical fiber guide, the tray having a fiber extraction opening for pulling the optical fiber onto the tray. A distance from the fiber extraction opening to a fixing position of the second optical fiber guide on the tray is set smaller than a distance from the fiber extraction opening to a fixing position of the first optical fiber guides on the tray.