Disclosed is an infusion flow regulator, in which a reference point is set within a manipulating range of a manipulation unit for regulating a flow rate in the infusion flow regulator, so that a manipulating point of the manipulation unit can be tuned with reference to the ratio of a flow rate measured at the reference point in relation to a prescribed injection flow rate, whereby the flow rate can be quickly and accurately tuned to the prescribed flow rate. The disclosure also includes an infusion flow regulating set including the infusion flow regulator, and an infusion flow regulating method using the same.