Invention Grant
US08741602B2 Method for producing glucuronic acid conjugate using Saccharomyces cerevisiae
有权
使用酿酒酵母生产葡萄糖醛酸结合物的方法
- Patent Title: Method for producing glucuronic acid conjugate using Saccharomyces cerevisiae
- Patent Title (中): 使用酿酒酵母生产葡萄糖醛酸结合物的方法
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Application No.: US13580316Application Date: 2011-02-14
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Publication No.: US08741602B2Publication Date: 2014-06-03
- Inventor: Shinichi Ikushiro , Toshiyuki Sakaki , Kaori Yasuda
- Applicant: Shinichi Ikushiro , Toshiyuki Sakaki , Kaori Yasuda
- Applicant Address: JP Toyama JP Toyama
- Assignee: Toyama Prefecture,Topu Bio Research Co., Ltd.
- Current Assignee: Toyama Prefecture,Topu Bio Research Co., Ltd.
- Current Assignee Address: JP Toyama JP Toyama
- Agency: Clark & Elbing LLP
- Agent Todd Armstrong
- Priority: JP2010-040150 20100225
- International Application: PCT/JP2011/053016 WO 20110214
- International Announcement: WO2011/105241 WO 20110901
- Main IPC: C12N15/81
- IPC: C12N15/81

Abstract:
Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using Saccharomyces pombe, and to provide a new means used in this production method. Disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is further inserted in a manner such that said gene can be expressed; and a method for producing a glucaronide that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronide of the aforementioned substance to be conjugated.
Public/Granted literature
- US20130059341A1 METHOD FOR PRODUCING GLUCURONIC ACID CONJUGATE USING SACCHAROMYCES CEREVISIAE Public/Granted day:2013-03-07
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