Invention Grant
US08741650B2 Methods for producing minus-strand RNA viral vectors using hybrid promoter comprising cytomegalovirus enhancer and chicken β-actin promoter
有权
使用包含巨细胞病毒增强子和鸡和肌动蛋白启动子的混合启动子产生负链RNA病毒载体的方法
- Patent Title: Methods for producing minus-strand RNA viral vectors using hybrid promoter comprising cytomegalovirus enhancer and chicken β-actin promoter
- Patent Title (中): 使用包含巨细胞病毒增强子和鸡和肌动蛋白启动子的混合启动子产生负链RNA病毒载体的方法
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Application No.: US10586142Application Date: 2005-01-20
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Publication No.: US08741650B2Publication Date: 2014-06-03
- Inventor: Akihiro Iida , Hiroshi Ban , Makoto Inoue , Takahiro Hirata , Mamoru Hasegawa
- Applicant: Akihiro Iida , Hiroshi Ban , Makoto Inoue , Takahiro Hirata , Mamoru Hasegawa
- Applicant Address: JP Tsukuba-shi, Ibaraki
- Assignee: DNAVEC Research Inc.
- Current Assignee: DNAVEC Research Inc.
- Current Assignee Address: JP Tsukuba-shi, Ibaraki
- Agency: Clark & Elbing LLP
- Priority: JP2004-014653 20040122
- International Application: PCT/JP2005/000705 WO 20050120
- International Announcement: WO2005/071092 WO 20050804
- Main IPC: C12N15/86
- IPC: C12N15/86
Abstract:
The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken β-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
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