Invention Grant
- Patent Title: Chemically Inducible Expression of Biosynthetic Pathways
- Patent Title (中): 生物合成途径的化学诱导表达
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Application No.: US12762941Application Date: 2010-04-19
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Publication No.: US08742203B2Publication Date: 2014-06-03
- Inventor: Lauralynn Kourtz , Oliver P. Peoples , Kristi D. Snell
- Applicant: Lauralynn Kourtz , Oliver P. Peoples , Kristi D. Snell
- Applicant Address: US MA Cambridge
- Assignee: Metabolix, Inc.
- Current Assignee: Metabolix, Inc.
- Current Assignee Address: US MA Cambridge
- Agency: Pabst Patent Group LLP
- Main IPC: C12N15/29
- IPC: C12N15/29 ; C12N15/31 ; C12N15/52 ; C12N15/82

Abstract:
Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.
Public/Granted literature
- US20100196974A1 Chemically Inducible Expression of Biosynthetic Pathways Public/Granted day:2010-08-05
Information query
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