The present invention provides an LED backlight driving circuit and an LED backlight driving method. The circuit includes an isolation MOS transistor, a dimming MOS transistor connected in parallel to the isolation MOS transistor, and first and second resistors. The first resistor and the second resistor are connected to each other in parallel and are connected between the dimming MOS transistor and a ground wire. The isolation MOS transistor and the dimming MOS transistor are arranged for electrical connection with an LED light. The LED backlight driving circuit arranges the isolation MOS transistor and the dimming MOS transistor to connect in parallel to each other so as to make the electrical current flowing through the isolation MOS transistor lessened to reduce the amount of heat emitting therefrom and enhance operation safety and also allow the MOS transistor used to be of a downgraded specification to lower down the manufacture cost.