Unwanted information contained in a reference image is reduced, thus acquiring a fluorescence image having quantitative intensity information, regardless of the angle and distance of excitation light. The invention provides a fluoroscopy apparatus including an illumination portion that irradiates an observation target with illumination light containing excitation light; a first image-acquisition section that acquires a fluorescence image in a prescribed observation region of the observation target; a second image-acquisition section that acquires an out-of-focus reference image of the observation target in the observation region; and an image correction section that corrects the fluorescence image acquired by the first image-acquisition section using the reference image acquired by the second image-acquisition section.