Invention Grant
- Patent Title: Method for production of polylactate using recombinant microorganism
- Patent Title (中): 使用重组微生物生产聚乳酸的方法
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Application No.: US13126157Application Date: 2009-10-27
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Publication No.: US08748137B2Publication Date: 2014-06-10
- Inventor: Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
- Applicant: Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
- Applicant Address: JP Aichi-ken
- Assignee: Toyota Jidosha Kabushiki Kaisha
- Current Assignee: Toyota Jidosha Kabushiki Kaisha
- Current Assignee Address: JP Aichi-ken
- Agency: Sughrue Mion, PLLC
- Priority: JP2008-276185 20081027
- International Application: PCT/JP2009/068402 WO 20091027
- International Announcement: WO2010/050470 WO 20100506
- Main IPC: C12P7/62
- IPC: C12P7/62 ; C12N9/00 ; C12N9/10 ; C12N1/20 ; C12N15/00 ; C07H21/04

Abstract:
It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
Public/Granted literature
- US20110212497A1 METHOD FOR PRODUCTION OF POLYLACTATE USING RECOMBINANT MICROORGANISM Public/Granted day:2011-09-01
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