Invention Grant
US08748152B1 Prevotella ruminicola xylose isomerase and co-expression with xylulokinase in yeast for xylose fermentation
有权
普罗维链霉菌木糖异构酶和酵母中的木酮糖激酶与木糖发酵的共表达
- Patent Title: Prevotella ruminicola xylose isomerase and co-expression with xylulokinase in yeast for xylose fermentation
- Patent Title (中): 普罗维链霉菌木糖异构酶和酵母中的木酮糖激酶与木糖发酵的共表达
-
Application No.: US13616629Application Date: 2012-09-14
-
Publication No.: US08748152B1Publication Date: 2014-06-10
- Inventor: Ronald E. Hector , Bruce S. Dien , Michael A. Cotta
- Applicant: Ronald E. Hector , Bruce S. Dien , Michael A. Cotta
- Applicant Address: US DC Washington
- Assignee: The United States of America, as represented by the Secretary of Agriculture
- Current Assignee: The United States of America, as represented by the Secretary of Agriculture
- Current Assignee Address: US DC Washington
- Agent Elizabeth R. Sampson; Randall E. Deck; John D. Fado
- Main IPC: C12N9/92
- IPC: C12N9/92
Abstract:
A xylose isomerase (XI) enzyme which exhibits increased activity and affinity for xylose is produced by strain TC2-24 of the rumen bacterium, Prevotella ruminicola. The gene encoding this enzyme may be used to produce improved recombinant yeast capable of utilizing xylose. The recombinant yeast are preferably transformed with heterologous polynucleotide sequences coding both the P. ruminicola XI, and the xylulokinase (XKS) of a Prevotella species. Yeast transformed with the polynucleotide sequences coding both of these XI and XKS exhibit significantly increased xylose utilization and cell growth on a culture medium containing xylose as the sole carbon source, in comparison to yeast transformed with XKS and XI from other sources.
Information query
