Apparatus to connect a flexible tube to a spigot on bio-disposable systems. The apparatus comprises a support for a spigot and jaws for gripping and holding a flexible tube. The jaws open and close for insertion of the flexible tube either under manual or pneumatic force. The jaws also include a front section that is openable against a resistance. In use, the end of the spigot is place inside the end of the flexible tube, the spigot is then positioned on the support, and the tube clamped into the jaws. Either on manual or pneumatic action, the spigot is advanced into the flexible tube within the jaws. The front section of the jaw opening slightly to accommodate the spigot, while the back section of the jaw holds the tube firmly in position.