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US08759034B2 Thermostable biocatalyst combination for nucleoside synthesis 有权
用于核苷合成的热稳定性生物催化剂组合

Thermostable biocatalyst combination for nucleoside synthesis
Abstract:
A recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterized in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNpase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
Abstract(Chinese):

重组表达载体，其包含：a）编码嘌呤核苷磷酸化酶（PNPase，E.C.2.4.2.1）的序列，b）编码尿苷磷酸化酶（UPase，E.C.2.4.2.3），c）或两者的序列; 每个序列与一个或多个控制序列可操作地连接，所述控制序列指导所述磷酸酶在合适的表达宿主中的产生; 所述源自古菌Thermoprotei类的序列，其特征在于PNPase来自硫酸梭菌（SEQ ID NO.7），UPase来自Aeropyrum pernix（SEQ ID NO.8）。 此外，本发明涉及在磷酸根离子存在下，在供糖核苷和受体碱之间的转糖基化方法，其特征在于所述方法包括使用Aeropyrum pernix的尿苷磷酸化酶（UPase）（NC_000854.2 ），硫唑硫酮的嘌呤核苷磷酸化酶（PNpase）（NC_002754.1），或其组合。

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