A new orthopedic prayer mat proposed, whereas the new orthopedic prayer mat has a multiple layered structure. The top layer is made from microfiber which is antimicrobial, anti-molding, water-resistant, anti-dust and heat-resistant. The bottom layer is an anti-slip layer to prevent the user from physical injury in any situation while using the mat, and a soft polyurethane layer and an anti-fatigue layer or a hard polyurethane layer are arranged between the top microfiber layer and the bottom anti-slip layer in order to absorb pressure pressure and providing support, and the soft and hard polyurethane layer are combined at an angle to adjust the ratio of the weight-absorption and bearing to form the orthopedic prayer mat. Further the angle between the layers is tailored for users according to user's weight, height, body structure type and healthy conditions.