Fluorescence detection apparatus detects fluorescence from a fluorescent object. The apparatus includes a light source configured to irradiate the fluorescent object with light, a shutter configured to block the light, from the light source, directed to the fluorescent object, an optical output measuring unit arranged in an optical path between the shutter and the light source, an image pickup element configured to detect the fluorescence from the fluorescent object and to capture a noise image, and a changing unit configured to change at least one of an accumulation time of the image pickup element and an open-close time of the shutter. The changing unit calculates the accumulation time for capturing the noise image using the measurement result of the optical output measuring unit, and corrects a captured fluorescent image generated by detecting the fluorescence, using the noise image captured during the accumulation time calculated by the calculation unit.