A touch panel frame structure is provided and includes a first frame including positioning grooves, a second frame covered by the first frame, a third frame, and an adhesive layer. The second frame includes first fixing portions located in the positioning grooves. The third frame includes a body portion, a supporting portion located on an inner edge of the body portion, and positioning boards. Each of the positioning boards is connected to the body portion and is coupled to one of the positioning grooves. Each of the positioning boards includes a plurality of second fixing portions. Each of the second fixing portions is fastened to one of the first fixing portions, such that each of the positioning boards is detachably fixed in one of the positioning grooves. The adhesive layer is located on the supporting portion to adhere a touch panel assembly.