Invention Grant
US08765370B2 Inhibition-based high-throughput screen strategy for cell clones
有权
用于细胞克隆的基于抑制的高通量屏幕策略
- Patent Title: Inhibition-based high-throughput screen strategy for cell clones
- Patent Title (中): 用于细胞克隆的基于抑制的高通量屏幕策略
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Application No.: US12792924Application Date: 2010-06-03
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Publication No.: US08765370B2Publication Date: 2014-07-01
- Inventor: Wei-Kuang Liu , Min-Pey Ding
- Applicant: Wei-Kuang Liu , Min-Pey Ding
- Applicant Address: TW
- Assignee: Scinopharm Taiwan, Ltd
- Current Assignee: Scinopharm Taiwan, Ltd
- Current Assignee Address: TW
- Agency: Andrews Kurth LLP
- Agent Aldo Noto; Michael Ye
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12N5/00 ; C07H21/04
Abstract:
A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell.
Public/Granted literature
- US20110124509A2 Inhibition-Based High-Throughput Screen Strategy for Cell Clones Public/Granted day:2011-05-26
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