Invention Grant
US08765448B2 Methods and means for exact replacement of target DNA in eukaryotic organisms
有权
在真核生物中精确替换靶DNA的方法和手段
- Patent Title: Methods and means for exact replacement of target DNA in eukaryotic organisms
- Patent Title (中): 在真核生物中精确替换靶DNA的方法和手段
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Application No.: US12663172Application Date: 2008-06-03
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Publication No.: US08765448B2Publication Date: 2014-07-01
- Inventor: Anne Rolland , Manuel Dubald , Michiel Van Lookeren Campagne , Rene Ruiter
- Applicant: Anne Rolland , Manuel Dubald , Michiel Van Lookeren Campagne , Rene Ruiter
- Applicant Address: BE Diegem
- Assignee: Bayer Cropscience, N.V.
- Current Assignee: Bayer Cropscience, N.V.
- Current Assignee Address: BE Diegem
- Agency: Hunton & Williams LLP
- Priority: EP07010998 20070605
- International Application: PCT/EP2008/004524 WO 20080603
- International Announcement: WO2008/148559 WO 20081211
- Main IPC: C12N1/00
- IPC: C12N1/00 ; C12N15/87 ; C12N15/74
Abstract:
Methods and means are provided for the exact exchange in eukaryotic cells, such as plant cells, of a target DNA sequence for a DNA sequence of interest through homologous recombination, whereby the selectable or screenable marker used during the homologous recombination phase for temporal selection of the gene replacement events can subsequently be removed without leaving a foot-print employing a method for the removal of a selected DNA flanked by two nucleotide sequences in direct repeats.
Public/Granted literature
- US20100175143A1 METHODS AND MEANS FOR EXACT REPLACEMENT OF TARGET DNA IN EUKARYOTIC ORGANISMS Public/Granted day:2010-07-08
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