A method of operating a multi-interface memory card including a first interface supporting a universal serial bus (USB) data transfer protocol and the second interface supporting an inter-chip USB (IC-USB) data transfer protocol includes; selecting either the first interface or the second interface to facilitate data communication between the multi-interface memory card and a host connected to the multi-interface memory card via a USB connection by comparing a level of a power-on voltage received from the host with a reference voltage level, and thereafter determining whether a reset signal has been received from the host.