A method and tools for virtually aligning object detection sensors on a vehicle without having to physically adjust the sensors. A sensor misalignment condition is detected during normal driving of a host vehicle by comparing different sensor readings to each other. At a vehicle service facility, the host vehicle is placed in an alignment target fixture, and alignment of all object detection sensors is compared to ground truth to determine alignment calibration parameters. Alignment calibration can be further refined by driving the host vehicle in a controlled environment following a leading vehicle. Final alignment calibration parameters are authorized and stored in system memory, and applications which use object detection data henceforth adjust the sensor readings according to the calibration parameters.