Invention Grant
US08778631B2 Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
有权
单体充电系统，用于使用体外蛋白质合成系统选择性地将非天然氨基酸引入蛋白质
- Patent Title: Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
- Patent Title (中): 单体充电系统，用于使用体外蛋白质合成系统选择性地将非天然氨基酸引入蛋白质
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Application No.: US12685795Application Date: 2010-01-12
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Publication No.: US08778631B2Publication Date: 2014-07-15
- Inventor: Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
- Applicant: Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
- Applicant Address: US CA South San Francisco
- Assignee: Sutro Biopharma, Inc.
- Current Assignee: Sutro Biopharma, Inc.
- Current Assignee Address: US CA South San Francisco
- Agency: Kilpatrick Townsend and Stockton LLP
- Main IPC: C07K1/00
- IPC: C07K1/00 ; C07K17/00
Abstract:
This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
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