An interface card quick plug-and-unplug device for use with a PCIe interface card is disclosed to include a shell covering a part of the PCIe interface card, and a locating member disposed at one side of the shell and/or the PCIe interface card for engagement with the PCIe slot upon insertion of the PCIe interface card into the PCIe slot. Biasing the locating member allows quick removal of the PCIe interface card from the PCIe slot without any tool. The interface card quick plug-and-unplug device enhances the flexibility of the design of computer circuit layout.