Invention Grant
US08808991B2 Ola-based methods for the detection of target nucleic avid sequences 有权
用于检测靶核酸序列的基于Ola的方法

  • Patent Title: Ola-based methods for the detection of target nucleic avid sequences
  • Patent Title (中): 用于检测靶核酸序列的基于Ola的方法
  • Application No.: US10570249
    Application Date: 2004-08-31
  • Publication No.: US08808991B2
    Publication Date: 2014-08-19
  • Inventor: René Cornelis Josephus Hodgers
  • Applicant: René Cornelis Josephus Hodgers
  • Applicant Address: NL Wageningen
  • Assignee: Keygene N.V.
  • Current Assignee: Keygene N.V.
  • Current Assignee Address: NL Wageningen
  • Agency: Foley & Lardner LLP
  • Agent Sunit Talapatra; Tianran Yan
  • Priority: NLPCT/NL03/00613 20030902; EP040766180 20040602
  • International Application: PCT/NL2004/000604 WO 20040831
  • International Announcement: WO2005/021794 WO 20050310
  • Main IPC: C12Q1/68
  • IPC: C12Q1/68 C12P19/34 C07H21/04
Ola-based methods for the detection of target nucleic avid sequences
Abstract:
Method for the detection of a target sequence comprising ligating two probes when annealed adjacent to the target sequence, hybridization of a compound primer to the ligated probes and after elongation of the compound primer, amplifying the elongated compound primer from primers annealing to primer binding sites provided in the compound primer and one of the probes to produce detectably amplicons.
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