In embodiments of wearable display device calibration, a first display lens system forms an image of an environment viewed through the first display lens system. A second display lens system also forms the image of the environment viewed through the second display lens system. The first display lens system emits a first reference beam and the second display lens system emits a second reference beam. The first display lens system then captures a reflection image of the first and second reference beams. The second display lens system also captures a reflection image of the first and second reference beams. An imaging application is implemented to compare the reflection images to determine a misalignment between the first and second display lens systems, and then apply an alignment adjustment to align the image of the environment formed by each of the first and second display lens systems.